Bold denotes added restriction sites and italics denote spacer nucleotides added to facilitate proper cutting of the sequence. Some primers used in the reporter assay constructs were situated upstream

NIAID Data Ecosystem20

Forward and reverse primers and the expected sizes of growth factors and β-actin.

PCR引物设计

基因表达分析

Forward and reverse primers and the expected sizes of growth factors and β-actin.

NIAID Data Ecosystem40

Nucleotide sequences of primers and PCR conditions used for RT-PCR and real-time PCR.

PCR引物设计

生物分子检测

R: RT-PCR; Q: Real-time PCR.

Figshare2015-12-02 更新20

Inner primers sequence nested PCR.

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