Construction of a SNP Fingerprinting Database and Population Genetic Analysis of Lithocarpus litseifolius (Hance) Chun Resources

Lithocarpus litseifolius (Hance) Chun, an evergreen plant of Fagaceae, commonly referred to as "sweet tea", is a good source of natural sweetener compounds. Additionally, the medicinal values of L. litseifolius have also been identified in recent year. In recent years, varietal identification has become a frequent problem in germplasm preservation collections, which causes considerable inconvenience and uncertainty in the cataloging and preservation of L. litseifolius germplasm resources, in the selection of parental lines for breeding, and in the promotion and use of high quality varieties. In this study, we used genotyping-by-sequencing (GBS) on 38 L. litseifolius accessions to develop SNP markers. After filtering, 13,883 high-quality SNPs were obtained. We used the SNPs to perform principal component analysis (PCA), population structure analysis, and neighbor joining (NJ) cluster analysis on the 38 L. litseifolius.