ChIP-seq of bovine cells (MDBK) to study butyrate-induced histone modification. Bos taurus
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA169504
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Utilizing next-generation sequencing technology, combined with ChIP (Chromatin Immunoprecipitation) technology to analyze histone modification (acetylation) induced by butyrate and to map the epigenomic landscape of normal histone H3, H4 Overall design: Cells were treated with 10 mM butyrate for 24 hr, The cells were scraped from the flask and homogenized with ice-cold Dounce homogenizer to release the nuclei. The collected nuclei were resuspended in digestion buffer and enzymatic shearing was performed. ChIP with anti H3, H4 and acetyl-H3 and acetyl-H4.
创建时间:
2012-06-27



