Supplementary Material for: Potentially Important MicroRNAs in Form-Deprivation Myopia Revealed by Bioinformatics Analysis of MicroRNA Profiling

Objective: This study aimed to identify differentially
expressed microRNAs (miRNAs) and to explore their potential roles in
form-deprivation myopia. Methods: The microarray data set
GSE58124 of miRNAs was downloaded from the Gene Expression Omnibus, and
form-deprivation myopia was induced in C57BL/6J mice over the right eye;
the contralateral, left eyes were used as controls. Differential
expression analysis was done using the LIMMA package. miRDB was used to
predict targets for miRNAs. The target genes were put into DAVID to
identify significant pathways and biological processes of miRNAs. A
functionally collaborative network was constructed using Cytoscape. Result:
In total, 24 and 20 upregulated miRNAs, respectively, were screened out
in retina and whole-eye tissue. However, there was no dramatic
expression change of miRNAs in sclera tissue. By taking intersections, 8
common upregulated miRNAs were obtained in both the retina and the
whole-eye samples. According to miRDB, 1,805 target genes were screened
out for the 8 differentially expressed miRNAs, including MAPK10
(mitogen-activated protein kinase 10). The functionally collaborative
network revealed that “regulation of transcription” was significantly
enriched. The pathways “Axon guidance” and “TGF-β signaling pathway”
were also enriched. Importantly, miR-466h-5p and miR-466j were
significantly enriched in some synaptic transmission-related biological
processes. Conclusion: This study identified an
upregulation of 8 miRNAs, which may function by disturbing their
enriched pathways or biological processes in the progression of myopia.