Adjusting ORG counts for low coverage genomes, or omitting low coverage species, improves correlation between RelCP and functional ORG number.

(a), Plot from figure 2b; RelCP vs. functional ORGs (log10). Blue circles: species with low coverage (≤ 6x) genome assemblies (r2 = 0.76, P < 0.0001). (b), Adjusted regression plot. Number of functional ORGs in low-coverage genomes is increased by 39.7%. Percent increase is derived by comparing the number of ORGs extracted from earlier, low-coverage draft genomes to the number from current ≥ 6x coverage assemblies, averaged over four available relevant cases (elephant, dog, cat, rabbit). Adjusted correlation: r2 = 0.84, P < 0.0001. Any increase in ORG count in low-coverage species strengthens the correlation, as all but two have relatively few ORGs for their RelCP. (c), Omitting species with ≤6x genomes strengthens the correlation between RelCP and functional ORGs further (r2 = 0.89, P < 0.0001).(a) 取自图2b的绘图：相对染色体概率（RelCP）与经log₁₀转换的功能型开放阅读框组（functional ORGs）的关系图。蓝色圆点代表基因组组装覆盖度≤6x的物种（决定系数r²=0.76，P<0.0001）。(b) 校正回归图。低覆盖度基因组中的功能型开放阅读框组数量提升了39.7%，该增幅通过对比4组相关案例（大象、犬、猫、兔）中，早期低覆盖度草图基因组提取的开放阅读框组数量与当前≥6x覆盖度组装基因组的对应数量的平均值计算得出。校正后的相关系数为r²=0.84，P<0.0001；由于除两个物种外，其余物种在对应RelCP下的功能型开放阅读框组数量均相对偏少，因此低覆盖度物种的开放阅读框组数量提升会进一步增强二者的相关性。(c) 剔除覆盖度≤6x的物种后，RelCP与功能型开放阅读框组之间的相关性进一步增强（r²=0.89，P<0.0001）。