RNA-seqs of SRSF3 and hnRNPK depleted C2C12 cells
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https://www.ncbi.nlm.nih.gov/sra/SRP292881
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Total RNA was extracted from C2C12 cells transfected with the indicated siRNA using TRIZOL (Thermo Fisher Scientific), and was further purified with Quick-RNA Miniprep Kit (Zymo research) according to the manufacturer's instructions. The extracted RNA was subjected to RNA-seq at Macrogen, Japan. Briefly, a sequencing library was prepared using the TruSeq Stranded mRNA kit (Illumina), and the library was read on Illumina NovaSeq 6000 (150 bp paired-end reads). Overall design: RNA-seqs of SRSF3 and hnRNPK depleted C2C12 cells
创建时间:
2021-10-20



