diabetes mellitus with tuberculosis RNA sequencing. Homo sapiens

Due to the rising prevalence and adverse effects of diabetes mellitus (DM), especially in countries where tuberculosis (TB) is most endemic, the DM-TB comorbidity has been recognized as a major obstacle towards achieving the World Host immune responses affected by diabetes can lead to increased susceptibility, severity and poor treatment outcomes in DM-TB patients, and the underlying mechanisms have not yet been fully elucidated. This study aimed to identify key immunological and cellular components that contribute to increased morbidity and mortality in DM-TB cases. We performed RNA-Seq of total RNA isolated from peripheral blood mononuclear cells (PBMCs) from 3 TB, 3 DM, and 3 DM-TB patients and healthy controls, and analyzed differential expression, pathway enrichment, and clustering of differentially-expressed genes (DEGs) to identify biological pathways altered specifically in DM-TB patients.