ChIP-seq and RNA-seq analysis during mononuclear phagocyte development

Mononuclear phagocytes including monocytes and dendritic cells are essential for immune responses against pathogens and tissue homeostasis. It has been well known that these cells are derived from bone marrow hematopoietic stem cells by passing through several myeloid progenitor populations. However, the molecular mechanisms underlying the fate decision of the mononuclear phagocytes remain unclear. In this project, we performed chromatin immunoprecipitation sequencing (ChIP-seq) and RNA-sequencing (RNA-seq) analysis of myeloid progenitor populations and mature mononuclear phagocytes to investigate the genome-wide epigenetic landscapes, transcription factor binding sites, and gene expression.