Subcellular localization of MAP6d1 and MAP6d1 mutants.
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NIH/3T3 cells were transfected with MAP6d1-myc or MAP6d1-myc mutants for 6 h, fixed using PFA-sucrose and immunolabeled with anti-myc antibody. At least 200 cells were analyzed and for each cell, the localization of MAP6d1 to microtubules, Golgi, plasma membrane and mitochondria has been determined. If no clear localization to one of these four subcellular compartments was found, the cell was quantified as “other”. Please note that there is more than one localization for MAP6d1 in some cells (as illustrated in Fig. 2A–C), such as Golgi and mitochondria or Golgi and microtubules. Subcellular localization of MAP6d1 and MAP6d1 mutants.
创建时间：
2014-12-19
