Genome-wide occupancy of EBF1-wt & EBF1-H240A. Mus musculus
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA345468
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资源简介：
To study the function of EBF1-H240 residue, genome-wide EBF1 ChIP was performed in A-MuLV transformed pro-B (Ebf1fl/fl;RERTCre) cells transduced with Ebf1wt and Ebf1H240A expressing vectors. Overall design: A-MuLV transformed Ebf1fl/fl RERTCre pro-B cells were treated with 2μM 4-hydroxy-tamoxifen (4-OHT) 24h after transduction. GFP+ cells were sorted two days after transduction and maintained in a 2μM 4-hydroxy-tamoxifen containing medium for 5 days and the replacement was validated. The cells were expanded further and used for ChIP analysis.
创建时间：
2016-10-05
