Seasonal Transcriptome Profile Across Three Different Tissues of the Andean Killifish Orestias ascotanensis

These data support the unpublished manuscript <i>"Seasonal Transcriptome Profile Across Three Different Tissues of the Andean Killifish Orestias ascotanensis"</i> (Maldonado et al., Manuscript ID: <code>SDATA-24-04380</code>).<b>1. Genome Assembly and Annotation</b><code><strong>Genome Assembly of Orestias ascotanensis.fa</strong></code>: Reference genome in FASTA format (originally published in <i>Di Genova et al. 2022</i>).<code><strong>annotation_Orestias (1).gtf</strong></code>: Structural annotation (GTF format) of gene models, used for RNA-seq alignment and transcript quantification.<code><strong>03_Gene_Annotation_Orestias_ascotanensis.csv</strong></code>: Detailed functional annotation of protein-coding genes (PCGs), including:Genomic coordinates (scaffold, start, end, strand).Gene/transcript IDs, assigned names, and protein products.Functional terms: InterPro domains, Gene Ontology (GO), KEGG Orthology (KO), and EC numbers.<b>2. Transcriptomic Data</b><code><strong>01_sample_info.csv</strong></code>: Metadata for RNA-seq samples (tissue, season, replicates, etc.).<code><strong>02_PCG_counts.csv</strong></code>: Raw read counts for all protein-coding genes across samples.<code><strong>04_DEGs.xlsx</strong></code>: Differentially expressed genes (DEGs) across conditions, with fold-change, <i>p</i>-values, and adjusted <i>p</i>-values.<b>3. Long Non-Coding RNA (lncRNA) Predictions</b><code><strong>Predicted_lncRNAs_Genomic_Locations_Orestias_ascotanensis.zip</strong></code>: BED files with genomic coordinates of lncRNAs predicted by four tools (CPAT, TransDecoder, FEELnc, RNAmining), including scaffold, coordinates, strand, and transcript IDs (where available).