Transfer RNAs (tRNAs) are key adaptor molecules in the protein translation machinery. In order to become fully active, they are heavily modified posttranscriptionally. Inosine at the first residue of
Here, we validate a novel protocol, Bulk RNA Barcoding and sequencing (BRB-seq), that combines the multiplexing-driven cost-effectiveness of a single-cell RNA-seq protocol with the efficiency of a bul