Figure S1 Expression of Sdt in embryogenesis.

(A) Extracts of 0-16h old embryos of either sdtK85 germ lines clones (mated with males carrying an FM7-ChFP balancer and sorted against ChFP) or wild type flies were blotted against Sdt and Actin. Both bands seen above the 100kDa marker band appear to be specific. (B) Extracts of staged embryos were blotted against Sdt and Actin. (C-D) Heterozygous and zygotic mutant sdtK85 embryos were stained with anti Sdt and DAPI. Note that the morphology of sdtK85 mutant embryos is already disturbed. Scale bars = 50µm.(A) 提取0至16小时龄的胚胎蛋白，样品来源分为两类：sdtK85生殖系克隆（与携带FM7-ChFP平衡子的雄性果蝇交配后，通过ChFP荧光信号分选获得）以及野生型果蝇；随后以Sdt和肌动蛋白（Actin）为靶标进行免疫印迹检测。在100kDa分子量标记条带上方出现的两条条带均为特异性条带。 (B) 提取不同发育阶段的胚胎蛋白，以Sdt和肌动蛋白为靶标进行免疫印迹检测。 (C-D) 对杂合型sdtK85突变胚胎和合子型sdtK85突变胚胎分别使用抗Sdt抗体与4',6-二脒基-2-苯基吲哚（DAPI）进行染色。可见sdtK85突变胚胎的形态已出现紊乱。标尺为50μm。