Effect of common mutations in clonal hematopoiesis of indeterminate potential (CHIP) on the transcriptome in bone marrow derived macrophages (BMDM).

Clonal hematopoiesis of indeterminate potential (CHIP) is associated with an increased risk of inflammatory conditions, including cardiovascular disease, in humans. The goal of the present data set was to examine the molecular mechanism underlying the increase in inflammation seen with the two most common CHIP mutations, namely loss-of-function mutations in Dnmt3a and Tet2, using murine bone-marrow derived macrophages (BMDM) as a model system. Overall design: We performed transcriptome analysis by RNAseq in bone-marrow derived macrophages from WT (Vav-Cre), Dnmt3aKO (Dnmt3afl/fl;Vav-Cre), Tet2KO (Tet2fl/fl;Vav-Cre) and Jak2VF (Jak2V617F/WT;Vav-Cre) mice, stimulated for 24h with either 200 mg/dL low-density lipoprotein (LDL) or vehicle control (ctl).