PrPSc in central nervous system, lympho-reticular system, mammary gland and milk duct lumen of natural scrapie exposed ewes bearing various genotypes at codons 136, 154 and 171 of the PRP gene.
收藏Figshare2015-12-02 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_PrP_Sc_in_central_nervous_system_lympho_reticular_system_mammary_gland_and_milk_duct_lumen_of_natural_scrapie_exposed_ewes_bearing_various_genotypes_at_codons_136_154_and_171_of_the_PRP_gene_/584476
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All investigated animals (n = 175) were PCR positive for Maedi lentivirus. Abnormal PrP (PrPSc) detection was carried out using Western Blotting (SHa31 anti-PrP antibody), ELISA (TeSeE Sheep and Goat, Bio-Rad) and immunohistochemistry (8G8 anti-PrP antibody). The majority of susceptible genotype sheep (ARQ/VRQ- VRQ/VRQ and ARQ/ARQ) were clinical suspect for scrapie at culling.
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2015-12-02



