PrPSc in central nervous system, lympho-reticular system, mammary gland and milk duct lumen of natural scrapie exposed ewes bearing various genotypes at codons 136, 154 and 171 of the PRP gene.

All investigated animals (n = 175) were PCR positive for Maedi lentivirus. Abnormal PrP (PrPSc) detection was carried out using Western Blotting (SHa31 anti-PrP antibody), ELISA (TeSeE Sheep and Goat, Bio-Rad) and immunohistochemistry (8G8 anti-PrP antibody). The majority of susceptible genotype sheep (ARQ/VRQ- VRQ/VRQ and ARQ/ARQ) were clinical suspect for scrapie at culling.