Optimized NHS-amine labeling for multiplexed phosphoproteome study.
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https://www.omicsdi.org/dataset/pride/PXD008082
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资源简介：
In this project, we report the detrimental effect of hydroxylamine, a quenching reagent for NHS-amine reaction, on phosphopeptides. The degree of phosphopeptide enrichment was greatly improved by a desalting method compared with a vacuum-drying method right after quenching reaction. We demonstrate that vacuum-drying in presence of hydroxylamine promotes β-elimination of phosphate groups from phosphoserine and phosphothreonine.
创建时间：
2018-02-27
