Expression data from rat NSCs co-cultured with green fluorescence protein (GFP)-ectodermal mesenchymal stem cells (EMSCs) versus mono-cultured rat NSCs

Rat NSCs were isolated from developing whole brian of the SD rat embryos at day 13.5. In the NSC/GFP-EMSC co-culture system, the NSCs showed significantly enhanced neuronal differentiation rather than astrocytic commitment, in comparison with the mono-cultured NSCs. In order to reveal the gene expression profiles of NSCs under these two diffferent culture conditions, we used microassrays to examine the global programme of gene expression of NSCs under these two diffferent culture conditions. These NSCs were co-cultured with GFP-EMSCs or mono-cultured for 14 days. The co-cultured NSCs were the expeimental group, while the mono-cultured ones were the control.