Investigating metabolism of artemisinin-resistant falciparum malaria parasites: mitochondrial rather than apicoplast pathways are key targets?

In the context of artemisinin resistance, the objective of the project was to evaluate the role of apicoplast and mitochondrion pathways during the parasite quiescent state induced by dihydroartemisinin (DHA) treatment. We performed gene expression profiling analysis using data obtained from RNA-seq of artemisinin-resistant parasites in two conditions: DHA treatment or DMSO treatment (as control condition) at the ring stage (obtained by D-sorbitol synchronization). Comparative gene expression profiling analysis of RNA-seq data for F32-ART (artemisinin-resistant) after 6 h treatment with 700 nM DHA compared to the control condition.