Mycorrhizal effects on glomalin-related soil protein and chlorophyll contents in coffee plants in the Peruvian Amazon

ABSTRACT The inoculation of vegetatively propagated coffee plants with arbuscular mycorrhizal fungi (AMF) prior to field establishment may positively influence the plant growth and physiology. This study aimed to evaluate the AMF effects on the glomalin-related soil protein and chlorophyll contents in vegetatively propagated coffee plants inoculated in a greenhouse and transplanted to an open field, in the Peruvian Amazon. The experiment consisted of eight treatments, in a 2 × 4 factorial design, being two coffee varieties (Caturra and Pache) and four AMF inocula (control, Moyobamba, El Dorado and Huallaga). The inocula were collected from organic coffee crops and named according to the province from which they were collected. The mycorrhizal colonization and chlorophyll content were statistically higher in the plants inoculated with AMF, if compared to the non-inoculated plants, while the glomalin-related soil protein content ranged from 61.6 to 69.1 mg g-1 and showed no statistically significant differences among the inocula, although the Moyobamba inoculum showed to be numerically superior. The effect of the coffee variety was not statistically significant among the variables under study.

摘要 本研究旨在探讨田间定植前对无性繁殖咖啡植株接种丛枝菌根真菌（arbuscular mycorrhizal fungi, AMF）的作用效果，该措施或可对植株生长及生理状态产生积极影响。本试验针对接种于温室后移栽至秘鲁亚马逊地区露天田地的无性繁殖咖啡植株，评估AMF对球囊霉素相关土壤蛋白及叶绿素含量的影响。试验采用2×4因子设计，共设置8个处理组，包含2个咖啡品种（卡杜拉Caturra、帕切Pache）与4种AMF接种剂（对照组、莫约班巴Moyobamba、埃尔多拉多El Dorado、瓦拉加Huallaga）。所有接种剂均采集自有机咖啡种植园，并以采集地所属省份命名。数据分析显示，与未接种植株相比，接种AMF的植株菌根定殖率与叶绿素含量均显著升高；球囊霉素相关土壤蛋白含量介于61.6至69.1 mg g⁻¹之间，尽管莫约班巴接种剂的数值略占优势，但各接种剂间未呈现统计学显著差异。此外，在所考察的变量中，咖啡品种的影响未达到统计学显著性水平。