Lnc-SLC15A1-1 Up-regulates CXCL10/CXCL8 Expression in Endothelial Cells by Sponging MicroRNAs (RNA-Seq)

Cardiovascular disease (CVD) is the leading cause of mortality in diabetes mellitus (DM). However, the molecular factors that cause this disproportiona increase in CVD in the DM/chronic kidney disease (CKD) population are still unknown.Human endothelial cells treated with high glucose to mimic DM and with the uremic toxin indoxyl sulfate (IS) to mimic the endothelial injury associated with CKD. Differentially expressed lncRNAs in these conditions were analyzed by RNA sequencing.Lnc-SLC15A1-1 expression was significantly increased upon IS treatment versus high glucose alone. Overall design: HUVECs were purchased from Lonza Walkersville, Inc. Cells from the fifth passage were incubated in 30 mM D-glucose (high glucose, HG) or 30 mM D-glucose treated with 0.1 mM IS (HG+IS) for 96 h.Total RNAs was extractied form the HUVECS with HG and HUVECs.Total RNA was extracted from cultured cells in these two conditions then RNA-seq was performed.