16S analysis of the project designed to study the role for gut microbiota in m6A/m epitranscriptomic mRNA modifications in different host tissues. We performed 4 different 16S analysis to investigate the most abundant genera of different mice cecal content.

In this study we performed MeRIP-Seq to study N6-methyl adenosine (m6A) modification of mRNA. We measured the effect of the microbiote on the transcriptomic and epi-transcriptomic regulation of Liver and Cecum mouse tissue. We performed 4 different 16S analysis: (i) Cecal content of CONV (n=13) and ex-GF (n=16) mice colonized with CONV flora for 4 weeks. (ii) Cecal content of vancomycin/amphotericin B-treated mice (n= 12) and abx mice that had been treated with vancomycin, metronidazol, neomycin, ampicillin and amphotericin B every 12h for 21 days (n=10, 3 independent experiments); CONV n=12; (iii) Cecal content of amphotericin B-treated mice (n= 5) and abx mice that had been treated with vancomycin, metronidazol, neomycin, ampicillin and amphotericin B every 12h for 21 days (n=5, experiment independent from data shown in ii); CONV n=5; (iv) Cecal content of mice mono-associated with A.muciniphila-, and L.plantarum- and E.coli Nissle. Ec mice displayed additional presence of an unknown member of the Lachnochlostridia.