Alteration of ileal lncRNAs after bariatric surgery is associated with regulation of lipid and amino acid metabolism

Metabolic and bariatric surgery (MBS) can generate a drastic shift of coding and non-coding RNAs expression pattern, which triggers organ function remodeling and may induce type 2 diabetes (T2D) remission. Our previous studies demonstrated that the altered expression profiles of duodenal and jejunal long noncoding RNAs (lncRNAs) after the duodenal-jejunal bypass (DJB), an investigational procedure and research tool of MBS, can improve glycemic control by modulating entero-pancreatic axis and gut-brain axis, respectively. As an indiscerptible part of intestine, the ileal lncRNAs expression signatures after DJB and the critical pathways associated with postoperative correction of impaired metabolism need to be investigated A cohort of male C57BL/6 mice were received from Shanghai Slyke Laboratory Animal Corporation (Shanghai, China). After one-week acclimatization, mice started to undergo high fat diet. At the end of 12 weeks HFD, 8 animals with typical diabetic phenotypes were selected and randomized into DJB or Sham groups based upon the procedure received.