Structural characterization of insoluble hydrophilic scaffolds for wound healing

Aim of the project is to assess the structural properties and evolution of scaffolds prepared by electro-spinning or force-spinning intended to enhance cutaneous wound healing of chronic lesions and burns. The composition and preparation protocol have been formulated in order to obtain scaffolds insoluble in aqueous fluids, suitable to support cell growth, migration and proliferation, while providing protection of the lesion from microbial contamination and an optimal hydration and gas exchange, crucial for healing. The scaffolds are based on biocompatible and bioactive natural polysaccharides, initially dissolved in water, with no need of any organic solvent, thus meeting the guidelines of green chemistry. We plan to compare the basic scaffolds, in the form of flat or tubular objects, with loaded ones, added with nanoparticles with phyllosilicates, hydroxyapatite, calcium carbonate and antimicrobials (ZnO NP, Ag, Cu NP) intended to improve the environment conditions for tissue repair.