Phosphoproteomic studies to identify targets and substrates of Protein kinase PfPK2 in human malaria parasite Plasmodium falciparum

The purpose of this project was to identify targets and substrates of Protein kinase PfPK2 in malaria parasite P. falciparum. These studies will help understand the mechanisms via which PfPK2, which is indispensable for the parasite survival, regulates parasite development. For this purpose, PfPK2-loxP parasite line was generated for conditional knock down of PfPK2 using rapamycin (RAP), which was used to induce the dimerization of active Cre-recombinase resulting in the depletion of PfPK2.