Allelic variation of BnaC.TT2.a and its association with seed coat color and fatty acids in rapeseed (Brassica napus L.)

Efficient molecular markers for the selection of rapeseed genetic materials with high seed oil content and ideal fatty acid (FA) composition are preferred by rapeseed breeders. Recently, we reported the molecular mechanism of TRANSPARENT TESTA 2 (TT2) in inhibiting seed FA biosynthesis in Arabidopsis. However, evidence showing the association of rapeseed TT2 homologs and seed FA production are still insufficient. In this study, we collected 83 rapeseed (Brassica napus L.) landraces from different geographical backgrounds to conduct association mapping of BnaC.TT2.a in relation to seed coat color and FA biosynthesis. Population background was corrected by 84 pairs of SSR markers that were uniformly distributed among the linkage groups of the Tapidor-Ningyou-7 DH population. A single copy of BnaC.TT2.a for single nucleotide polymorphism (SNP) assay was cloned by a pair of previously reported specific primers. From the analysis of BnaC.TT2.a allelic variations using GLM+Q model, four SNPs ...油菜育种工作者亟需高效分子标记，以筛选种子含油量高且脂肪酸（fatty acid, FA）组成理想的油菜遗传材料。此前本团队已报道拟南芥中透明种皮2（TRANSPARENT TESTA 2, TT2）抑制种子脂肪酸生物合成的分子机制，但目前关于油菜TT2同源基因与种子脂肪酸生成关联的研究证据仍较为匮乏。本研究收集了83份来自不同地理背景的甘蓝型油菜（Brassica napus L.）地方品种，针对BnaC.TT2.a基因开展种皮颜色与脂肪酸生物合成的关联分析。以均匀分布于Tapidor-Ningyou-7双单倍体（Doubled Haploid, DH）群体连锁群的84对简单序列重复（Simple Sequence Repeat, SSR）标记校正群体遗传背景。通过一对已报道的特异性引物克隆得到可用于单核苷酸多态性（Single Nucleotide Polymorphism, SNP）分析的单拷贝BnaC.TT2.a基因。利用GLM+Q模型对BnaC.TT2.a的等位基因变异进行分析，共得到4个SNP位点……