Comparison of gene expression between wild-type and Osterix1-Cre:Hdac3 CKO BMSC-derived osteoblasts
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Bone marrow stromal cells (BMSC) were isolated from femurs and tibias of littermate wildtype and CKO mice. Cells were plated in osteogenic culture medium (alphaMEM, 20% FBS, 1% antibiotic/antimycotic, 1% non-essential amino acids, 50 ug/mL ascorbic acid, 10 mM beta glycerophosphate, 10^-7 M dexamethasone) and cultured for 7 days prior to RNA isolation. Total RNA isolated from osteogenic cultures of BMSC grown for 7 days
创建时间：
2019-10-07
