Expression data of resident macrophages from the livers of wildtype mice and Melanocortin 4 receptor-deficient (MC4R-KO) mice with non alcoholic steatohepatitis (NASH)

There is a unique histological structure termed hepatic crown-like structure (hCLS), in which CD11c-positive macrophages surround dead or dying hepatocytes with large lipid droplets in NASH livers. To characterize gene expression patterns, we performed microarray analysis using hepatic resident macrophages. CD11c-positive macrophages exhibited distinct polarization profiles from typical M1 and M2 macrophages, and there were clear differences in the gene expression profiles from CD11c-negative macrophages. F4/80 highly expressed resident macrophages were sorted based on the expression levels of CD11c from the livers of wildtype mice fed standard diet (SD) (CD11c-negative) and MC4R-KO mice fed Western diet (WD) for 20 weeks (CD11c-negative and positive).