Optimizing NG-CanCan Protocol Delivery with MHz Compression to Minimize Near-Electrode Ablation

This study advanced the Next Generation (NG) CanCan protocols by implementing MHz compression for electric pulse packet delivery. Packets were delivered at 0.2 MHz, and the compression significantly reduced near-electrode cell ablation compared to delivery at 1 Hz, while preserving effects at the center. The experiments used a four-electrode array, with YoPro-1 to evaluate cell permeabilization and Propidium Iodide to assess cell death two hours post-delivery. This work was supported by NIH grant 1R21EY034258.