Discovery and characterization of single nucleotide polymorphisms in coho salmon, Oncorhynchus kisutch

Molecular population genetic analyses have become an integral part of ecological investigation and population monitoring for conservation and management. Microsatellites have been the molecular marker of choice for such applications over the last several decades, but single nucleotide polymorphism (SNP) markers are rapidly expanding beyond model organisms. Coho salmon (Oncorhynchus kisutch) is native to the north Pacific Ocean and its tributaries, where it is the focus of intensive fishery and conservation activities. As it is an anadromous species, coho salmon typically migrate across multiple jurisdictional boundaries, complicating management and requiring shared data collection methods. Here, we describe the discovery and validation of a suite of novel SNPs and associated genotyping assays which can be used in the genetic analyses of this species. These assays include 91 that are polymorphic in the species and one that discriminates it from a sister species, Chinook salmon. We demons...分子群体遗传学分析已成为保护与管理相关的生态学调查及种群监测工作中不可或缺的组成部分。近数十年来，微卫星（Microsatellite）始终是此类研究首选的分子标记，但近年来单核苷酸多态性（single nucleotide polymorphism，SNP）标记的应用范围正快速突破模式生物的局限。银鲑（Coho salmon，Oncorhynchus kisutch）原产于北太平洋及其支流流域，是高强度渔业活动与保护工作的重点关注对象。由于该物种为溯河洄游性鱼类，通常会跨多个行政管辖区域洄游，这给管理工作带来了复杂性，也要求采用统一的数据采集方法。本研究报道了一套全新的SNP标记及其配套的基因分型检测方法，可用于该物种的遗传学分析。该套检测方法包含91个可在银鲑中呈现多态性的标记，以及1个可将其与近缘物种奇努克鲑（Chinook salmon）区分开来的标记。本研究证实……