TGFβ signaling in the uterine epithelium controls endometrial cell homeostasis and regeneration [CUT&RUN]

In this study, we define the epithelial-specific contribution of SMAD2/3 to endometrial function by conditionally ablating the Smad2 and Smad3 transcription factors using Lactoferrin-iCre (Ltf-cre). Using epithelial organoid cultures from the endometrium, we uncover the signaling mechanisms downstream of TGFβ that control endometrial cell regeneration and are critical for endometrial regeneration and homeostasis. We performed Cleavage Under Targets & Release Using Nuclease (CUT&RUN-seq) for SMAD4 in the mouse endometrial epithelium organoids.