Per-animal raw data, Western-blot source data, and reagent inventories supporting "Phytochemically Standardised Aqueous Hellenia speciosa Rhizome Extract is Associated with Attenuated HFD/STZ-Induced Diabetic Hepatic Injury in Male Wistar Rats"

This dataset provides the per-animal raw values, Western-blot source-data files, histopathology source images, HPLC-DAD raw chromatograms, reagent inventories, and statistical-analysis outputs underlying the summary statistics reported in the manuscript referenced below.

The study evaluated a phytochemically standardised aqueous rhizome extract of Hellenia speciosa (J.Koenig) S.R.Dutta (synonyms: Costus speciosus; Cheilocostus speciosus; Costaceae; abbreviated CS-AE) in a six-arm preclinical design (n = 10 male Wistar rats per group): NC, NC + CS-AE, DC, DC + Metformin, DC + CS-AE, DC + MET + CS-AE. Diabetes was induced by 4 weeks of high-fat diet followed by a single low-dose streptozotocin injection (35 mg/kg). The six-week treatment phase quantified metabolic biochemistry, hepatic oxidative stress, ex vivo substrate-supported mitochondrial bioenergetics, Western-blot densitometry of the Nrf2/Keap1, AMPK/PGC-1a, mitochondrial-dynamics, and apoptosis axes, qRT-PCR (single-reference Gapdh plus dual-reference Gapdh + Hprt1 sensitivity re-analysis), NAS-adapted histopathology, Masson's trichrome morphometry, and immunohistochemistry for nuclear Nrf2, HO-1, and cleaved caspase-3.

The deposit is structured as follows:
01_Per_Animal_Data: Supplementary Dataset S1 (15 worksheets)
02_Western_Blot_SourceData: full-membrane TIFFs, per-target densitometry, ROI overlays, processing methods
03_Histopathology_Photomicrographs: 5 stains x 60 animals x 5 fields per animal (1500 source images)
04_HPLC_Chromatograms: Agilent ChemStation .D folders for each batch, stability time-point, and reference standard
05_Inventories: antibody and TaqMan inventory spreadsheets
06_Statistical_Outputs: GraphPad Prism .pzfx, R scripts, ANOVA / ART-ANOVA / Spearman / geNorm output PDFs
07_qPCR_Raw_Ct_Values: QuantStudio .eds and .xlsx exports

ARRIVE 2.0, ICH Q2(R2), and ConPhyMP / Journal of Ethnopharmacology best-practice reporting. Ethics approval HAPO-02-K-012-2025-05-2711 (Umm Al-Qura University BREC).

本数据集提供了下述参考文献稿件中报告的汇总统计数据所依托的每只动物原始数值、蛋白质免疫印迹（Western-blot）原始源数据文件、组织病理学原始图像、高效液相色谱-二极管阵列检测器（HPLC-DAD）原始色谱图、试剂库存清单以及统计分析输出结果。

本研究采用六组临床前实验设计（每组n=10只雄性Wistar大鼠），评估了经植物化学标准化的艳山姜（Hellenia speciosa (J.Koenig) S.R.Dutta，异名：Costus speciosus；Cheilocostus speciosus；Costaceae，缩写为CS-AE）根茎水提物。实验分组为：正常对照组（NC）、正常对照组+CS-AE组、糖尿病模型组（DC）、糖尿病模型组+二甲双胍组、糖尿病模型组+CS-AE组、糖尿病模型组+二甲双胍+CS-AE组。糖尿病造模方式为：先给予4周高脂饮食，随后单次低剂量链脲佐菌素（35 mg/kg）注射。为期6周的治疗阶段中，检测指标包括代谢生化指标、肝脏氧化应激水平、离体底物支持的线粒体生物能学、Nrf2/Keap1、AMPK/PGC-1α、线粒体动力学以及细胞凋亡通路的蛋白质免疫印迹光密度分析、定量实时聚合酶链式反应（qRT-PCR，以单内参Gapdh以及双内参Gapdh+Hprt1进行敏感性重分析）、美国国立卫生研究院卒中量表（NAS）适配的组织病理学评估、马松三色染色形态计量分析，以及针对核Nrf2、HO-1和剪切型半胱氨酸天冬氨酸蛋白酶-3（cleaved caspase-3）的免疫组化检测。

本数据集的存储结构如下：
01_Per_Animal_Data：补充数据集S1（共15个工作表）
02_Western_Blot_SourceData：完整膜蛋白质免疫印迹TIFF图像、各靶标光密度数据、感兴趣区域（ROI）叠加图、处理方法文件
03_Histopathology_Photomicrographs：5种染色方式 × 60只动物 × 每只动物5个视野（共计1500张原始图像）
04_HPLC_Chromatograms：对应每一批次、稳定时间点以及参考标准品的安捷伦化学工作站（Agilent ChemStation）.D格式文件夹
05_Inventories：抗体与TaqMan探针库存清单电子表格
06_Statistical_Outputs：GraphPad Prism .pzfx格式文件、R脚本、方差分析（ANOVA）/对齐秩变换方差分析（ART-ANOVA）/斯皮尔曼相关分析/geNorm分析结果PDF文件
07_qPCR_Raw_Ct_Values：QuantStudio .eds与.xlsx格式导出文件

本研究遵循ARRIVE 2.0、ICH Q2(R2)以及《ConPhyMP》《Journal of Ethnopharmacology》（民族药理学杂志）的最佳报告规范。伦理审批编号为HAPO-02-K-012-2025-05-2711（乌姆古拉大学生物研究伦理委员会）。