Towards a human brain EV atlas: Characteristics of EVs from different brain regions, including small RNA and protein profiles

Brain derived EVs (bdEVs) from eight brain regions, including orbitofrontal gyrus(ORB), postcentral gyrus (POSTC), hippocampus (HIPPO), thalamus (THAL), occipital gyrus (OCC), medulla (MED), corpus callosum (CORP), and cerebellum (CBLM) were separated and characterized by small RNA sequencing. We aim to provide an atlas of bdEV molecular profiles with brain region specificity. RNA was extracted from bdEVs of eight brain regions and small RNA libraries construction by the D-Plex Small RNA-seq Kit (Diagenode C05030001). Sequencing data of bdEVs from eight different regions were then compared.