New activity of Astaxanthin Data

Network pharmacology methods were used to analyze the targets and signaling pathways of astaxanthin in the treatment of spermatogenesis. An in vitro cell culture method was used to observe the effects of astaxanthin on the proliferation and apoptosis of spermatogonial stem cells. The therapeutic effect of astaxanthin on oligoasthenozoospermia in mice induced with cyclophosphamide was observed. ACSL3, VDAC, GPX4, FADS2, GLS2, Steap3, MDA, GSH-Px, iron ions, etc., were detected. The role and mechanism of these were analyzed. The results showed Astaxanthin has 52 key targets for treating spermatogenesis, among which the oxidative stress metabolic pathway is one of the most important factors. The sperm concentration and forward motility of the model mice fed with astaxanthin were significantly greater than those of the control group. The proliferation rate of spermatogonial stem cells cultured with astaxanthin was also significantly greater than that of the control group, and the proportion of apoptotic cells was significantly lower. Astaxanthin can reduce ACSL3, MDA, and iron ions in spermatogonial stem cells; increase the expression of Steap3, VDAC, GPX4, GLS2, GSH-Px, and FADS2; and improve ester metabolism to promote spermatogenesis in oligoasthenozoospermia model mice.