遇见数据集

Bayou virus screening data of marsh rice rats (Oryzomys palustris) trapped on oiled and unoiled plots in Plaquemines Parish, Louisiana, 2013-06-03 to 2017-07-02

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DataONE2025-02-04 更新2025-04-26 收录
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Marsh rice rats were captured and dissected in June 2013-2017 at four oiled, three unoiled, and three reference sites. Blood was collected from mark and release animals, and a subset of rice rats were dissected for tissue samples. Total RNA was isolated from lung and kidney tissues so that viral RNA (belonging to a hantavirus strain, called Bayou virus) might be detected by qRT-PCR. Whole blood or serum samples were screened by immunofluorescent assays to detect antibodies for hantaviruses. In addition to this data, sample collection information is also reported.

2013年至2017年6月,研究人员在4个石油污染样地、3个未受石油污染样地及3个对照样地捕获沼泽稻鼠(Marsh rice rat)并实施解剖。从标记-放归个体采集血液样本,同时选取部分稻鼠解剖以获取组织样本。研究人员从肺与肾组织中提取总RNA,通过qRT-PCR(实时定量反转录聚合酶链反应)检测属于巴约病毒(Bayou virus)的汉坦病毒(hantavirus)毒株的病毒RNA。采用免疫荧光分析法对全血或血清样本进行筛查,以检测汉坦病毒抗体。本数据集除上述检测数据外,亦同步报告了样本采集相关信息。

创建时间:
2025-02-05
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