Data on genetic diversity of circumsporozoite protein (csp) non-repeat regions from Plasmodium knowlesi clinical isolates of Sabah.

This dataset presents an analysis genetic diversity of malaria circumsporozoite protein (csp) of Plasmodium knowlesi in Sabah; where circumsporozoite protein is one of the targeted candidates for malaria vaccine development and was conducted to evaluate the suitability of csp as a vaccine in relation to its genetic diversity. The data were collected from 26 human blood spot samples from Kudat and Kota Kinabalu hospitals in Sabah in 2012 which were tested positive for malaria. Genomic DNA extraction, nested PCR, cloning and sequencing of the csp genes were carried out and phylogenetic, sequence diversity and natural selection of the csp genes were analysed using bioinformatic tools such as MEGAX and DnaSP ver. 5.10.00 for phylogenetic tree build, mutational analysis and neutral theory of evolution. Analysis and comparison of this gene was done against P. knowlesi csp strain H as a reference sequence (GenBank database XM_002258966.1) showed point mutations at 52 positions among the 237 sequences of different geographical regions. The phylogenetic tree revealed that the occurrence of multiple haplotypes was scattered despite of geographical location. The evolutionary history which was inferred using the Neighbor-Joining method revealed no geographical clustering to any country listed above; with a total of 76 non-repeat region Pkcsp haplotypes including one unique haplotype (haplotype H12). These data could serve as auxiliary information and/or research data for other researchers in Sabah. It could also serve as guide or reference data to other researchers outside Sabah who may be interested in carrying out similar research in other states.

本数据集展示了马来西亚沙巴地区疟原虫恶性疟（Plasmodium knowlesi）子孢子蛋白（csp）的遗传多样性分析；子孢子蛋白是疟疾疫苗研发的热门候选分子之一，本研究旨在评估csp作为疫苗的适用性及其与遗传多样性的关系。数据来源于2012年沙巴库达特和哥打京那巴鲁医院的26份人类血斑样本，这些样本经检测确诊为疟疾阳性。通过基因组DNA提取、巢式PCR、csp基因克隆和测序等方法，对csp基因进行了分析。利用MEGAX和DnaSP ver. 5.10.00等生物信息学工具对csp基因进行了系统发育分析、序列多样性和自然选择分析。将本基因分析结果与恶性疟csp参考菌株H（GenBank数据库XM_002258966.1）进行比对，发现在237个不同地理区域的序列中存在52个位置的点突变。系统发育树显示，尽管地理分布不同，多等位基因的出现仍呈散布状。通过邻接法推断的进化历史表明，未发现与上述任何国家相关的地理聚类；共包含76个非重复区域Pkcsp等位基因，包括一个独特的等位基因（等位基因H12）。这些数据可为沙巴地区的研究人员提供辅助信息和/或研究数据，同时也可作为沙巴以外研究人员开展类似研究的指南或参考数据。