Supplementary data

STATA output from the statistical analysis of the research project "Human native skin fibroblast proliferation enhancement in natural salso-bromo-iodic mineral water added in-vitro culture" carried out at the University of Pavia (Italy).

The favourable, regenerative effects on wound healing of some mineral waters have long been empirically demonstrated.The aim of this experimental study was to investigate the effects of an Italian salso-bromo-iodic mineral water (Rivanazzano, PV, Italy) on an in-vitro human native fibroblast culture model, in order to point out any potential regenerative action.Human native fibroblasts were cultured in different experimental conditions:−    Dulbecco’s Modified Eagle’s Medium powder (DMEM) reconstituted with distilled water (Control).−    DMEM reconstituted with filtered mineral water collected at the spring.−    DMEM reconstituted with filtered mineral water collected at the balneotherapy facility.−    DMEM reconstituted with filtered heated mineral water collected at the balneotherapy facility.−    DMEM partially replaced with filtered mineral water collected at the spring at different concentrations (10%, 20%, 30%, 40%, 50%).−    DMEM partially replaced with filtered heated mineral water collected at the balneotherapy facility at different concentrations (10%, 20%, 30%, 40%, 50%).−    DMEM partially replaced with filtered mineral water collected at the balneotherapy facility at different concentrations (10%, 20%, 30%, 40%, 50%).Cell proliferation and viability were evaluated using spectrophotometric analysis after cell staining with the XTT Microculture Tetrazolium Assay. A statistical analysis was performed for each experimental condition at 24, 48 and 72 hours.The best outcome was demonstrated in the fibroblasts cultured with DMEM partially replaced with filtered mineral water collected at the spring within the range of 20-50%.The results from our research showed a stimulating activity of the Rivanazzano salso-bromo-iodic mineral water on in-vitro human native fibroblast cultures. This activity was the strongest in the water collected at the spring, while it decreased in the water collected at the balneotherapy facilities. These results could be the baseline for clinical applications within the field of wound healing and balneotherapy.