Expression data from JVM13 cells with cyclin D1 exogenous overexpression

Mantle cell lymphoma (MCL) is an aggressive B-cell neoplasm characterized by the t(11;14)(q13;q32) translocation leading to cyclin D1 overexpression. Cyclin D1 is a major cell cycle regulator and also has a role in transcription, but the effect of the latter in tumorigenesis remains largely unknown. Here, we investigated the transcriptional role of cyclin D1 in MCL and its impact on the pathogenesis of this neoplasm. Integrating genome-wide expression analysis of cyclin D1-silenced and overexpressing cells with cyclin D1 chromatin binding profiles, we identified a cyclin D1-activated transcriptional program in MCL cells. We used microarrays to analyze the genome-wide expression modulation in cyclin D1 overexpression models established in the cyclin D1-negative lymphoblastoid cell line JVM13. We generated cyclin D1 inducible overexpression models in JVM13, with wild type cyclin D1 or a highly stable form of the protein with the T286A mutation. Control cells were generated in parallel with the corresponding empty vector. We then treated the cells with doxycycline to induce the expression of cyclin D1 and we extracted RNA to performed expression microarrays.