Additional file 4 of Development of a CRISPR/Cpf1 system for targeted gene disruption in Aspergillus aculeatus TBRC 277

Additional file 4: Fig. S4. Transformation efficiency of A. aculeatus TBRC 277 in the presence or absence of Cpf1 endonuclease containing plasmids. To investigate the toxicity of Cpf1 on the A. aculeatus host, 10-μg DNA of each plasmids were independently transformed into TBRC 277 protoplast. The number of transformants were recovered from minimal medium (MM+Czapek-Dox+bleomycin+sorbitol) supplemented with Uri/Ura. Protoplast transformed with empty vector, pCRISPR01, has no FnCpf1 gene (dark grey); protoplast transformed with FnCpf1-containing plasmid, pCRISPR01-FnCpf1 (light-grey); protoplast transformed with FnCpf1 and crRNA-pyrGs, pCRISPR01-FnCpf1-pyrGs (pyrG-1, pyrG-2, or pyrG-3) (white). The graph shows the means and standard deviation (SD) from two independent experiments.

附加文件4：图S4. 在存在或不存在Cpf1内切酶含有的质粒的情况下，A. aculeatus TBRC 277的转化效率。为探究Cpf1对A. aculeatus宿主的毒性，将每种质粒的10-μg DNA独立转化至TBRC 277原生质体。转化子数量从含有Uri/Ura的最低培养基（MM+Czapek-Dox+博来霉素+山梨醇）中回收。以空载体pCRISPR01转化的原生质体中不含有FnCpf1基因（深灰色）；含有FnCpf1基因的质粒pCRISPR01-FnCpf1转化的原生质体（浅灰色）；同时含有FnCpf1和crRNA-pyrGs的质粒pCRISPR01-FnCpf1-pyrGs（pyrG-1、pyrG-2或pyrG-3）转化的原生质体（白色）。图表展示了两次独立实验的平均值和标准差（SD）。