Figure_5_replicates

(G-I) Viral RNA was extracted from viral stocks (G), Xrn1 knock out A549 cells 16 hours post-infection (H), or 48-hour supernatants from infected MDCK cells (H). Viruses analyzed: WT PR8 and PR8-PA(∆X) strains with no insert, or PR8 with WT or mutant STOML2 sequences inserted in the WT PR8 or PR8-PA(∆X) background. Mutant STOML2 sequences contain the GCUG to UAGC mutation preventing PA-X cleavage. Viral RNA was reverse-transcribed and PCR-amplified using primers shown as black arrows in F-G to determine whether the STOML2 sequences were retained. R# --> replicate number; LG### --> experiment number .sgd are the original source files obtained with the Syngene Imager