Heavy chain immunoglobulin sequencing of Lyme disease PBMCs

Lyme disease (Borrelia burgdorferi infection) is increasingly recognized as a significant source of morbidity world-wide. Here, we investigated B cell responses to Lyme disease through molecular identifier-enabled antibody heavy chain sequencing of bulk B cells from PBMCs. Single-cell immunoglobulin sequencing of paired heavy- and light-chain genes from this project will also be separately deposited. Additional information regarding patient characteristics and overlap with other data from the SLICE study is available upon request. Total mRNA was isolated from peripheral blood mononuclear cells (PBMCs) of Lyme disease patients at four timepoints: Visit 1, Untreated acute infection; Visit 3, one month after completion of treatment; Visit 5, six months after treatment; Visit 7, two years after treatment. Total mRNA was isolated from peripheral blood mononuclear cells (PBMCs) of Healthy controls at three timepoints: Visit H2, initial visit; Visit H3, six months after initial; Visit H4, one year after initial visit. cDNA was tagged with unique molecular identifiers, amplified with primers specific for immunoglobulin constant regions, and sequenced by HiSeq 2500.