RNA sequencing analysis of WT and Ythdf2-KO FGSCs

YTHDF2 displays extensive-expression patterns during oocyte maturation and its deficiency causes female infertility in mice. However, its specific mechanism of regulation remains elusive due to the absence of suitable in vitro models. Female germline stem cells (FGSCs) possess the capacity for self-renewal and differentiation into oocytes to support reproduction. The successful establishment of a line of FGSCs provides a platform for scientific research on female fertility and oogenesis. To understand how YTHDF2 exerts its regulatory effects on FGSCs, we conducted RNA-seq assay in WT and Ythdf2-KO FGSCs and aimed to identified YTHDF2-responsive genes.