SOM3B Analysis of fresh healthy human bone marrow

To assess the global activity of DNA, RNA, and protein synthesis in individual bone marrow cells. In parallel, cell type specific markers were measured to characterize these activities in major developmental sub-populations present during hematopoiesis in healthy human bone marrow.
Conclusion:
Healthy bone marrow immune cells demonstrate variable activity of DNA, RNA, and protein synthesis, dependent on cell identity, proliferation, and developmental timing. Of note, we observe a the highly proliferative erythroid lineage is among the populations with highest RNA and protein synthesis. Interestingly, we also observe in plasma cells, differentiated B cells tasked with maintaining humoral immunity with antibody production, a high activity of protein synthesis in the absence of de novo RNA synthesis. Antibodies used in this experiment are validated and titrated using positive and negative control cell lines and/or primary immune samples. Control experiments blocking incorporation of precursor metabolic molecules confirmed specificity of their labeling in human bone marrow cells.