Transcriptional effects of chronic methamphetamine treatment on mouse myocardium

In this study, we constructed a mouse model of methamphetamine-associated cardiomyopathy (MAC). We gave C57Bl/6 mice 10 mg/kg of methamphetamine for 4 weeks. Echocardiographic and myocardial pathological staining revealed hypertrophic and fibrotic remodeling of the myocardium, left ventricular dilatation, and left ventricular ejection fraction decreasing (%LVEFExperimental design: Male C57BL/6 mice were given intraperitoneal injections of methamphetamine dissolved in sterile saline twice daily at 12-hour intervals for 4 weeks. Control mice were injected with sterile saline on the same schedule. After the experiment, the hearts were excised and RNA was extracted from the left ventricular myocardial tissue. Transcript dysregulation was identified in the hearts of mice that responded to methamphetamine exposure.