Controls applied in 16S rRNA RT/IS2404 qPCR.
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Table 4 indicates controls applied in 16S rRNA RT/IS2404 qPCR. NA, not applicable.a16S rRNA RT PCR, reverse transcriptase real-time PCR targeting the 16S ribosomal RNA of M. ulcerans.bIS2404 qPCR, quantitative real-time PCR targeting the insertion sequence (IS) 2404 of M. ulcerans.cgDNA, genomic DNA wipeout was conducted using DNAses provided in the QuantiTect Reverse Transcription Kit (Qiagen).dTaqMan exogenous internal positive control (Applied Biosystems, Carlsbad, CA).ecDNA, complementary DNA obtained through reverse transcription of M. ulcerans RNA by QuantiTect Reverse Transcription Kit (Qiagen).
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2015-12-02
