Nonclassical beta catenin signaling regulate skeletal cell fate determination

To rigorously examine the role of b-catenin dependent transcription, we created mice deficient for the transcriptional output of beta-catenin in skeletal mesenchyme (bcat-dTF-Dermo1-cKO) and compare the transcriptional profiles to that of the beta-catenin conditional KO mice in skeletal mesenchyme (bcat-Dermo1-cKO). Total RNAs were isolated from calvarial mesenchyme at E15.5 and followed by comparative gene expression profiling analysis of RNA-seq data for control, bcat-dTF-Dermo1-cKO, and bcat-Dermo1-cKO.