a New Marine-Derived Asterosaponin (CN-3) alleviated the proliferation of U251 MG cells. a New Marine-Derived Asterosaponin (CN-3) alleviated the proliferation of U251 MG cells

Analysis of U251 glioblastoma multiforme (GBM) cells treated with a new culcita novaeguineae asterosaponion, CN-3. A new asterosaponin was isolated from culcita novaeguineae, an abundant marine resource in the south China sea. The asterosaponin induced significant growth inhibition with a 50% inhibitory concentration at 48 h of 2.013 μg/mL in U251MG cells. 1.8μg/mL of the asterosaponin reduced U251 MG cells viability from 100 % to 42.5% (24 h), 37.4% (48 h) and 52.1% (72 h). In this study, a microarray analysis was performed using RNA prepared from U251MG GBM cells treated with the asterosaponion. These data revealed that 661 genes had significant differential expressions. Overall design: U251 cells were treated with asterosaponin or control, At 48h, total RNA were extracted for microarray experiment. Four replicates were performed of asterosaponion, and two replicates were performed of control.