SeqTrace_Human.zip

This dataset consists of scanning electron microscopy (SEM) measurements and sequencing data of two population specific variants; rs11150606 (chr16:g.31087690T>C (hg38) p.Gln30Arg) observed in East Asian (EAS) and Native and Latin Americans and rs201075024 (chr16:g.31087679C>T (hg38 p.Gly34Ser) which is observed exclusively in South Asians (SAS) in PRSS53. These variants are thought to influence hair shape. The samples were subsequently sequenced for the EDAR variant rs3827760 (chr2:g.108897145G>C (hg38p.Val370Ala).Malaysians (sample size: 99) from different ethnicities were recruited for this study and hair and saliva samples were collected from all the participants, after obtaining informed consent. DNA extracted from saliva samples was genotyped by sequencing a fragment of the PRSS53 gene and EDAR gene. The samples then underwent hair phenotype assessment using a scanning electron microscope (SEM). The images captured were measured for several parameters using ImageJ, and the measurements were tabulated. The SEM images and sequencing files are available with this data set. Mouse hair samples were obtained from University of Edinburgh and Sanger Institute. These samples consist of a knockout, SAS specific variant and EAS specific variant strains, for the PRSS53 variants. Auchene hair samples were used for the SEM analysis, where the images were analysed with ImageJ, and the data tabulated.

本数据集包含PRSS53基因上两类群体特异性变异的扫描电子显微镜（scanning electron microscopy，SEM）测量数据与测序数据：其一为rs11150606（chr16:g.31087690T>C（hg38）p.Gln30Arg），在东亚（East Asian，EAS）及美洲原住民与拉丁美洲人群中检出；其二为rs201075024（chr16:g.31087679C>T（hg38）p.Gly34Ser），仅在南亚（South Asian，SAS）人群中检出。上述变异被认为可影响毛发形态。
本研究招募了不同族裔的马来西亚受试者（样本量：99例），在获取所有参与者的知情同意后，收集其毛发与唾液样本。通过对PRSS53基因与EDAR基因的片段进行测序，对唾液样本提取的DNA开展基因分型。随后使用扫描电子显微镜（SEM）对样本开展毛发表型评估，利用ImageJ软件对拍摄的图像进行多项参数测量，并将测量结果整理为表格。本数据集附带SEM图像与测序文件。
小鼠毛发样本取自爱丁堡大学与桑格研究所，涵盖针对PRSS53基因变异的敲除品系、南亚特异性变异品系与东亚特异性变异品系。使用小鼠耳廓毛发样本开展SEM分析，通过ImageJ完成图像分析并将数据整理为表格。