Identification of differentially expressed mRNAs after activation of LX-2 cells

In this study, high-throughput RNA sequencing technology combined with bioinformatics analysis was used to compare the differentially expressed mRNA of TGF-ß1-treated LX-2 cells with that of the control group in hopes of gaining a deeper understanding of the biological function and signaling pathway changes in the activation process of LX-2 cells, as well as to identify potential key genes regulating HSC activation. Overall design: LX-2 cells were divided into two groups. The experimental group received 10 ng/ml TGF-ß1 stimulation (n=3), while the control group received no treatment (n=3). After 24 hours, the levels of a-SMA gene and protein expression were measured by PCR and Wb, respectively, to demonstrate that the cells had been activated. Total RNA was sequenced by transcriptome to screening for differentially expressed genes (DEGs), and DEGs were bioinformatically analyzed.