Assessing UV-B induced DNA damage in Antarctic plants: is desiccation a compounding factor?

Metadata record for data from ASAC Project 1310See the link below for public details on this project.---- Public Summary from Project ----Increasing UV-radiation over Antarctica each spring may damage DNA in plants. This research determined the susceptibility of Antarctic plants to such damage and investigated the effectiveness of protective and repair mechanisms. This helps predict how plants globally will cope with future climate change.Samples have been collected from Heard Island, and near Casey Station, Antarctica. Three excel files constitute this dataset.Heard Island 2003/4 Samples collected for project 1310Vascular plant UVB siteGPS coordinates of vascular plant UV site 53 06 57 S, 73 43 30E A total of 4 g of each of 5 species (Pringlea antiscorbutica, Poa cookii. Deschampsia antarctica. Azorella selago, Acaena magellanica) were collected on 5 days over the season. These were analysed for DNA damage, UVB absorbing pigments, and photosynthetic and photoprotective pigments. Chlorophyll fluorescence and leaf temperature were measured on the sampled plants.Nutrient gradientGPS co-ordinates: high nutrient site: 53 06 0.419S, 73 43 0.105E 4g of P. antiscorbutica and 0.8g of P. cookii were taken at the high nutrient site, along with chlorophyll fluorescence measurements.GPS co-ordinates: low nutrient site: 53 06 29.09S, 73 43 00.36E 7.2g of P. antiscorbutica and 3.2g of P. cookii were taken at the low nutrient site, along with chlorophyll fluorescence measurements.Scarlett HillGPS co-ordinates: 53 05 0.645S, 073 40 0.339E3.2 g of P. antiscorbutica plants was sampled and chlorophyll fluorescence measurements taken.Spit CampGPS co-ordinates:3.2g of P. antiscorbutica plants was sampled and chlorophyll fluorescence measurements taken.Ceratodon Paddick ValleyGPS co-ordinates: 53 08 43.44S, 73 40 35.42E 3 g of Ceratodon purpureus was collected.The fields in this dataset are:SpeciesDateWeightgrams dry weight (gdw)Water ContentAspectCommunity typeTime (Local Time)FluorescenceLeaf temperature (1, 2, etc)F - Chlorophyll FluorescenceFm' - Fluorescence maximum measured in the lightYield - Yield of fluorescenceNo. and Mark are stamps put on the data during download

本元数据记录来自ASAC项目1310的相关数据，项目公开详情可参见下方链接。
---- 项目公开摘要 ----
每年春季南极上空的紫外线辐射强度持续升高，可能会对植物的DNA造成损伤。本研究明确了南极植物对这类损伤的易感程度，并探究了防护与修复机制的有效性，该研究结果有助于预测全球植物如何应对未来气候变化。

样本采集于赫德岛（Heard Island）以及南极洲凯西站（Casey Station）附近区域。本数据集包含3个Excel文件：
1. 赫德岛2003/2004年项目1310采集样本
- 维管植物UVB采样点：GPS坐标为南纬53°06′57″、东经73°43′30″。本研究于该观测季的5个采样日内，分别采集了5个物种的样本（每份重量约4g），分别为：根菜菜（Pringlea antiscorbutica）、库克早熟禾（Poa cookii）、南极发草（Deschampsia antarctica）、阿佐雷拉（Azorella selago）、麦哲伦龙牙草（Acaena magellanica）。对样本开展了DNA损伤、紫外线B（UVB）吸收色素以及光合与光保护色素相关分析，并对采样植株测定了叶绿素荧光与叶片温度。
- 营养梯度采样：
高营养位点GPS坐标：南纬53°06′0.419″、东经73°43′0.105″。在此位点采集了4g根菜菜（Pringlea antiscorbutica）与0.8g库克早熟禾（Poa cookii），并同步开展叶绿素荧光测定。
低营养位点GPS坐标：南纬53°06′29.09″、东经73°43′00.36″。在此位点采集了7.2g根菜菜（Pringlea antiscorbutica）与3.2g库克早熟禾（Poa cookii），并同步开展叶绿素荧光测定。
- 斯嘉丽山（Scarlett Hill）采样：GPS坐标为南纬53°05′0.645″、东经73°40′0.339″。采集了3.2g根菜菜（Pringlea antiscorbutica）植株，并开展叶绿素荧光测定。
- 斯皮特营地（Spit Camp）采样：未标注GPS坐标，采集了3.2g根菜菜（Pringlea antiscorbutica）植株，并开展叶绿素荧光测定。
- 帕迪克谷（Paddick Valley）紫萼藓采样：GPS坐标为南纬53°08′43.44″、东经73°40′35.42″。采集了3g紫萼藓（Ceratodon purpureus）样本。

本数据集包含以下字段：
物种（Species）、采样日期（Date）、重量（Weight，单位为克干重gdw）、含水量（Water Content）、坡向（Aspect）、群落类型（Community type）、当地时间（Time (Local Time)）、荧光（Fluorescence）、叶片温度（1、2等，代表重复测量编号）、F - 叶绿素荧光（F - Chlorophyll Fluorescence）、Fm' - 光下测定的最大荧光（Fm' - Fluorescence maximum measured in the light）、荧光产量（Yield - Yield of fluorescence）、编号与标记（No. and Mark，为数据下载过程中添加的标识）。